【文獻標(biāo)題】Low-functional programming of the CREB/BDNF/TrkB pathway mediates cognitive impairment in male offspring after prenatal dexamethasone exposure
【作者】Wanting Dong，Dan Xu， Zewen Hu，et.al
【作者單位】武漢大學(xué)（Wuhan University）
【文獻中引用產(chǎn)品】
試劑-二乙醚
【關(guān)鍵詞】Pregnancy dexamethasone exposure,Hippocampus,Cognitive impairment,Glucocorticoid receptor,Brain-derived neurotrophic factor
【DOI】https://doi.org/10.1016/j.toxlet.2017.10.020
【影響因子(IF)】5.70
【出版期刊】《Toxicology Letters》
【產(chǎn)品原文引用】
Specific pathogen-free Wistar rats (No. 2015-0018, certification
number: 42000600014526, licence number: SCXK (Hubei), with weights of 200 ± 20 g for females ａnd 280 ± 20 g for males) were obtained from the Experimental Center of the Hubei Medical Scientific Academy (Wuhan, China). Animal experiments were performed at the Center for Animal Experiments of Wuhan University (Wuhan, China),which has been accredited by the Association for Assessment ａnd Accreditation of Laboratory Animal Care International (AAALAC International). The Committee on the Ethics of Animal Experiments of the Wuhan University School of Medicine approved the protocol (permit number: 201719). All animal experimental procedures were performed in accordance with the Guidelines for the Care ａnd Use of Laboratory Animals of the Chinese Animal Welfare Committee.Animals were housed in cages with padding in an air-conditioned room under standard conditions (room temperature: 18–22 °C; humidity: 40%–60%; light cycle: 12 h light-dark cycle; 10–15 air changes per hour) ａnd allowed free access to rat chow ａnd tap water. All rats were acclimated one week before experimentation, ａnd two female rats were then placed together with one male rat overnight in a cage for mating. The appearance of sperm in the vaginal smears confirmed mating, ａnd the day of mating was set as gestational day (GD) 0. As shown in Fig. 1, a group of pregnant rats received 0.2 mg/kg examethasone (No. H42020019, Shuanghe Pharmaceutical Company,Wuhan, China) subcutaneously every day starting from GD9. On GD20,the pregnant rats were anaesthetized using diethyl ether (No.10009318, Hengyuan Biotech Co., Ltd., Shanghai, China) ａnd sacrificed. Pregnant rats with litters sizes of 12–14 (the male/female ratio was approximately 1:1) were considered qualified (n = 14 pregnant rats per group), ａnd male fetal rats were collected. Four whole brains were randomly collected for each group ａnd fixed in 10% formalin,paraffin embedded ａnd sectioned. The hippocampal samples that were dissected ａnd collected from each littermate were pooled together ａnd immediately frozen in liquid nitrogen for subsequent analyses. Another group of pregnant rats were allowed to deliver spontaneously at term.The number of pups per litter was randomly selected to 8 to ensure adequate ａnd equal nutrition until weaning. At postnatal week 4 (PW4),
10 male pups from 10 different mothers were selected randomly for each group. At PW26, all pups were subjected to behavioural tests.After the tests, all rats were anaesthetized ａnd sacrificed to collect the whole brain or hippocampal tissues.
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