【文獻(xiàn)標(biāo)題】Phosphate regulates malate/citrate-mediated iron uptake ａnd transport in apple

【作者】Jiu-Cheng Zhang，Xiao-Na Wang，Wei Sun，et.al

【作者單位】山東農(nóng)業(yè)大學(xué)（Shandong Agricultural University）

【文獻(xiàn)中引用產(chǎn)品】

植物蘋果酸(MALIC ACID)elisa試劑盒

植物檸檬酸（Citric Acid）ELISA試劑盒

【關(guān)鍵詞】Low phosphate，Iron-deficiency，Absorption，Organic acids，Apple

【DOI】https://doi.org/10.1016/j.plantsci.2020.110526

【影響因子(IF)】5.90

【出版期刊】《Plant Science》

【產(chǎn)品原文引用】

Rhizosphere organic acid efflux collection ａnd measurement

The apple plants were treated in hydroponic nutrient solution for 4 days (pH = 5.8), ａnd the nutrient solution was then dried to a powder in a -80℃ freeze dryer. The powder was dissolved with 5 ml of deionized water, the solution was then passed through a cation exchange resin ａnd the filtrate was collected. After lyophilizing again, the sample was dissolved in 5 ml of deionized water ａnd stored at -20℃. The organic acid efflux was analyzed via enzyme-linked immunosorbent assay (ELISA; Hengyuan, Shanghai, China). Organic acid was made into a solid-phase antibody, ａnd horseradish peroxidase (HRP) was then combined with the organic acid antibody to form an antibody-antigenenzyme-labeled antibody complex. After thorough washing, the TMB substrate was added to develop the color, with the TMB converted to blue under the catalysis of HRP ａnd finally converted to yellow under the action of acid. The shade of the color positively correlated with the organic acid concentration in the sample. Finally, the absorbance was measured using a microplate reader at a wavelength of 450 nm. The organic acid concentration was calculated from a standard curve.Effluent malate content = (337.57 ×A450 –9.0216) ×5 ×V / (134.09× M). Effluent citrate content = (506.82 ×A450 –17.148) ×5 ×V /(192.13 × M). (A: absorbance, V: volume of filtrate, M: fresh weight of apple root).

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原創(chuàng)作者：上海恒遠(yuǎn)生物技術(shù)發(fā)展有限公司