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  五月，大家的論文寫得怎么樣了？
先我們要祝賀下來自南京農(nóng)業(yè)大學(xué)的王老師，2021年王老師在《International Journal of Nanomedicine》（納米醫(yī)學(xué)雜志）發(fā)表了SCI文獻(xiàn)，發(fā)表的文獻(xiàn)影響因子(IF)達(dá)到了5.15分！然后讓我們恭喜王老師到了恒遠(yuǎn)生物的獎學(xué)金！

這篇論文寫的非常棒，其中的很多實(shí)驗(yàn)方法和思路值得我們閱讀，小編想要分享給大家，希望能夠?qū)ψ鰧?shí)驗(yàn)的小伙伴們有點(diǎn)點(diǎn)幫助，也希望大家積的參與我們的“發(fā)論文贏獎學(xué)金”活動，小編等著你們的投稿哦，下個獎學(xué)金的人說不定就是你！

恒遠(yuǎn)產(chǎn)品文獻(xiàn)分享：

【文獻(xiàn)標(biāo)題】
Nanoparticles of Chitosan/Poly(D,L-Lactide-CoGlycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice
【作者單位】南京農(nóng)業(yè)大學(xué)（Nanjing Agricultural University）
【文獻(xiàn)中引用產(chǎn)品】
小鼠免疫球蛋白G1(IgG1)elisa試劑盒
小鼠IgG2a抗體亞型elisa試劑盒
小鼠免疫球蛋白M(IgM)elisa試劑盒
小鼠白介素4(IL-4)ELISA試劑盒
小鼠白介素12(IL-12)ELISA試劑盒
小鼠白介素17A(IL-17A)ELISA試劑盒
小鼠γ干擾素(IFN-γ)ELISA試劑盒
小鼠轉(zhuǎn)化生長因子β（TGF-β） ELISA試劑盒
【關(guān)鍵詞】
【DOI】https://doi.org/10.2147/IJN.S301851
【影響因子(IF)】5.15
【出版期刊】《International Journal of Nanomedicine》

二、產(chǎn)品原文引用

Cytokine Assays
The levels of IL-4, IL-12, IL-17, IFN-γ, ａnd TGF-β in sera from the ten groups were investigated with commercial ELISA kits (HengYuan, Shanghai, China) according to the manufacturer’s guidelines.

Characterization of rHCA59-CS NPs ａnd rHCA59-PLGA NPs
After conjugation, PLGA ａnd Chitosan nanoparticles were precipitated ａnd the free amounts of rHCA59 protein were determined by a BCA protein assay kit. It was found that 89% ａnd 71% of rHCA59 were conjugated to PLGA ａnd Chitosan, respectively. In other words, a total of 1.78 mg of protein bound to PLGA, ａnd 1.42 mg of protein bound to Chitosan.A scanning electron microscopy was used to identify the size ａnd morphology of PLGA ａnd Chitosan nanoparticles. The SEM results showed that both PLGA ａnd Chitosan nanoparticles’ appearances were smooth. The size of rHCA59-PLGA NPs was from 180 nm to 402 nm (Figure 1A) ａnd the size of rHCA59-CS NPs was from 200 nm to 500 nm (Figure 1B).

Figure 3 Effects of different antigen delivery systems on the expressions of multiple cytokines. Sera were collected ａnd detected on day 14 by ELISA.
Notes: (1): PBS group (blank control). (2): pET-32a protein group. (3): PBS-PLGA group. (4): PBS-CS group. (5): PBS-CFA group. (6): rHCA59 group. (7): rHCA59-PLGA group. (8): rHCA59-CS group. (9): rHCA59-CFA group. (10): rHCA59-CS-PLGA group. (A) IL-12. (B) IL-17. (C) IFN-γ. (D) IL-4. (E) TGF-β. Data are representative of triplicate independent experiments (*p < 0.05, **p < 0.01, ａnd ***p < 0.001). Abbreviations: PLGA, poly-D,L-lactide-co-glycolide; CS, Chitosan; CFA, complete Freund’s adjuvant; IL, interleukin; ELISA, enzyme linked immunosorbent assay

Figure 5 Effects of different antigen delivery systems on T lymphocyte subsets.
Notes: rHCA59 loaded nanoparticles could affect the proportions of CD4+ T cells ａnd CD8+ T cells in immunized mice. The percentages of CD4+ T cells (A1–A10) ａnd CD8+ T cells (B1–B10) in ten groups were investigated by flow cytometry on day 14 after immunization. (C ａnd D) Different treatments affected the proportions of CD4+ T cells ａnd CD8+ T cells. (A1 ａnd B1) PBS group (blank control). (A2 ａnd B2) PBS-CFA group. (A3 ａnd B3) PBS-PLGA group. (A4 ａnd B4) PBS-CS group. (A5 ａnd B5) pET-32a protein group. (A6 ａnd B6) rHCA59 group. (A7 ａnd B7) rHCA59-CFA group. (A8 ａnd B8) rHCA59-CS group. (A9 ａnd B9) rHCA59-PLGA group. (A10 ａnd B10) rHCA59-CS-PLGA group. The results shown here are from three independent experiments (*p < 0.05, **p < 0.01, ａnd ***p < 0.001). Abbreviations: CD4, cluster of differentiation 4; CD8, cluster of differentiation 8; T cells, thymus cells; PLGA, poly-D,L-lactide-co-glycolide; CS, Chitosan; CFA, complete Freund’s adjuvant.